By Lyn Healy, Ludmila Ruban
This lavishly-illustrated, authoritative atlas explores the complicated artwork of culturing human pluripotent stem cells. Twelve chapters – containing greater than 280 colour illustrations – conceal quite a few issues in pluripotent stem mobilephone culturing together with mouse and human fibroblasts, human embryonic stem cells and caused pluripotent stem cells, attribute staining styles, and irregular cultures, between others. Atlas of Human Pluripotent Stem Cells in tradition is a accomplished selection of illustrated concepts complemented by way of informative and academic captions studying what high quality cells seem like and the way they behave in quite a few environments. Examples of excellent cultures are in comparison side-by-side to less-than-perfect and unacceptable examples of human embryonic and brought about pluripotent stem mobilephone colonies. This certain and thorough atlas is a useful source for researchers, academics, and scholars who're attracted to or operating with stem mobilephone culturing.
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Extra info for Atlas of Human Pluripotent Stem Cells in Culture
Cryopreservation and quality controls of mouse embryonic feeder cells. Cryobiology. 2011;63:104–10. Eiselleova L, Peterkova I, Neradil J, Slaninova I, Hampl A, Dvorak P. Comparative examine of mouse and human feeder cells for human embryonic stem cells. Int J Dev Biol. 2008;52:353–63. Fernandes AM, Meletti T, Guimarães R, Stelling MP, Marinho PA, Valladão AS, Rehen SK. world wide survey of released strategies to tradition human embryonic stem cells. telephone Transplant. 2010;19:509–23. Lee JB, tune JM, Lee JE, Park JH, Kim SJ, Kang SM, et al. on hand human feeder cells for the upkeep of human embryonic stem cells. replica. 2004;128:727–35. Leonardo TR, Schell JP, Tran HT, Peterson SE. instruction of mouse embryonic fibroblast feeder cells. In: Loring JF, Peterson SE, three Inactivated Mouse and Human Fibroblasts editors. Human stem cellphone guide. 2d ed. London: Elsevier; 2012. p. 15–27. Michalska AE. Isolation and propagation of mouse embryonic fibroblasts and training of mouse embryonic feeder layer cells. Curr Protoc Stem mobilephone Biol. 2007;3:1C. three. 1–17. Nieto A, Cabrera CM, Catalina P, Cobo F, Barnie A, Cortés JL, et al. impression of mitomycin-C on human foreskin fibroblasts used as feeders in human embryonic stem cells: immunocytochemistry MIB1 rating and DNA ploidy and apoptosis evaluated by means of movement cytometry. telephone Biol Int. 2007;31:269–78. Prathalingam N, Ferguson L, younger L, Lietz G, Oldershaw R, Healy L, et al. creation and validation of an outstanding production perform grade human fibroblast line for helping human embryonic stem phone derivation and tradition. Stem mobilephone Res Ther. 2012;3:12. Roy A, Krzykwa E, Lemieux R, Néron S. elevated potency of gamma-irradiated as opposed to mitomycin C-treated feeder cells for the growth of standard human cells in long term cultures. J Hematother Stem mobilephone Res. 2001;10:873–80. Unger C, Felldin U, Nordenskjöld A, Dilber MS, Hovatta O. Derivation of human epidermis fibroblast traces for feeder cells of human embryonic stem cells. Curr Protoc Stem cellphone Biol. 2008;5:1C. 7. 1–10. four Human Embryonic Stem Cells In 1998, the derivation of the 1st 3 human embryonic stem mobile (hESC) traces from the interior telephone mass of a blastocyst was once suggested. The cells grew as colonies and have been derived and maintained on mouse feeder cells. The cells displayed an ordinary karyotype over the said eight months in non-stop mobilephone tradition and displayed cellphone floor markers attribute of undifferentiated non-human primate embryonic stem telephone strains (Chap. eight) and human embryonal carcinoma cells. those cells have been proven to be pluripotent in nature via their skill to shape teratomas in immunocompromised mice, composed of tissues derived from all 3 germ layers. the capability utilisation of those cells for illness modelling, toxicology experiences and regenerative drugs heralded a brand new period within the box of biology. The regimen tradition of demonstrated human mobilephone strains within the laboratory less than the fundamental rules of fine mobile tradition perform (GCCP) is considered undemanding; often, their tradition stipulations are powerful, as are their morphological features.